*This authors were equally contributed to this article.
Our aim was to evaluate the changes in blood glucose control and lipid profiles after 2-months of smoking cessation in healthy males.
Smoking abstinence was evaluated through self-report and urine cotinine levels. 12 individuals who succeeded in quitting smoking were analyzed. Fasting values of glucose and insulin were used to estimate the β-cell activity and insulin resistance was evaluated using the Homeostasis Model Assessment (HOMA) and Quantitative Insulin Sensitivity Check Index (QUICKI).
The data showed that the subjects had a significant increase in weight, body mass index and fasting plasma glucose levels after smoking cessation. The HOMA-Insulin Resistance and the HOMA β-cell function increased significantly (p=0.005, p=0.047 respectively). The QUICKI showed a significant decrease (p=0.005). In addition, the low-density lipoprotein cholesterol levels decreased significantly (p=0.028); however, changes in the high-density lipoprotein cholesterol, the triglyceride and total cholesterol levels were not significant (p=0.284, p=0.445 respectively).
During the initial stage of smoking abstinence, insulin resistance increased and insulin sensitivity decreased due to elevated body weight and fat composition. Therefore, it is important to educate individuals that stop smoking about the necessity of weight control during smoking cessation programs.
Smoking is known to negatively impact human health. Carcinogenic processes, vasomotor dysfunction, impaired endothelial-dependent vasodilatation, and the modification of lipid profiles are included in these adverse effects.
Although the underlying mechanism is not clear, Kong et al.
Smoking has also been shown to be associated with insulin resistance in both non-diabetic
Whether smokers have worse lipid profiles than non-smokers is controversial. A meta-analysis of 54 cross-sectional studies with respect to the association of lipid profiles and cigarette smoking status revealed that smokers had higher serum concentrations of total cholesterol (TC) (3.0%), TG (9.1%), very-low-density lipoprotein cholesterol (10.4%), and LDL-C (1.7%), and lower serum concentrations of HDL-C (5.7%) and apolipoprotein A1 (4.2%) than nonsmokers. In addition, a meta-an-alysis of 27 prospective studies, on the effects of smoking cessation on lipid profiles revealed that smoking cessation significantly increased the level of HDL-C but not of TC, LDL-C, or TG.
Twenty male volunteers 28-52 years (mean age of 39 years) were enrolled from among the hospital staff at the Bucheon St. Mary's hospital at The Catholic University of Korea via advertisements in the hospital newspapers and website. All of the participants fulfilled the Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition, Text Revision (DSM-IV-TR) criteria for nicotine dependence. The DSM-IV-TR diagnoses were determined by two board-certified psychiatrists. All participants abstained from illegal drugs and other medications and none had mental or physical disorders except for nicotine dependence. None of the healthy volunteers had a family history of psychiatric illness or medical problems. As a part of the initial clinical evaluation, all of the smokers were asked to complete baseline questionnaires requesting detailed information regarding demographics and smoking-related clinical variables. The severity of nicotine dependence was evaluated by the Fagerström Test for Nicotine Dependence (FTND).
During the study period, smoking abstinence was evaluated through self-report and urine cotinine levels. The self-report was assessed based on the responses to the question Have you quit smoking since you enrolled in this study? The urine cotinine levels of all subjects were monitored three times: before cessation, one month after, and two months after smoking cessation. Urinary samples were obtained without prior warning. The urine cotinine levels were measured by a urinary cotinine immunoassay dipstick (Nicometer, Jant Pharmaceutical, CA, USA). Among 20 participants, 12 smokers succeeded in quitting smoking (validated by a urine cotinine level ≤40 ng/mL) and eight smokers failed. Therefore, only the 12 successful individuals were analyzed.
Written informed consent was obtained from each subject after they had been given a complete explanation of the study. The protocol was approved by the Bucheon St. Mary's Hospital ethics committee of the Catholic University of Korea and all procedures conformed to the Helsinki Declaration of 1975, as revised in 1983.
Blood was drawn from the participants twice, at baseline and 2 months after smoking cessation. At 7:00 a.m., following an overnight fast, a sample of blood was collected from all subjects to measure the plasma glucose, HDL-C, LDL-C, TC, TG, total protein and albumin levels. The plasma glucose, TC, TG, HDL-C, and LDL-C levels were analyzed using enzymatic techniques (Choongwae Pharma., CO., Seoul, Korea) with a Hitachi 7600-110 analyzer. Thyroid stimulating hormone (TSH) concentrations were analyzed by immunoradiometric assay using an IRMA-mat TSH (IZOTOP, The Institute of Isotopes Co., Ltd., Budapest, Hungary) with RIA-mat-280 equipment. Triiodothyronine (T3) levels were analyzed using RIA-mat-280 equipment with the RIA-mat-T3 (Diasorin, Stillwater, USA). Free thyroxine (free T4) levels were analyzed using RIA-mat-280 equipment with the RIA-FT4 (Immunotech, Radiova, Prague, Czech Republic). Growth hormone levels were analyzed using immunoradiometric assay techniques (Daiichi,Tokyo, Japan) in a 1,470 wizard γ-counter. Somatomedin-C levels were analyzed by radioimmunoassay (BioSource Europe S.A., Belgium) in a 1,470 wizard γ-counter. Adrenocorticotropic hormone (ACTH) levels were measured by immunoradiometric assay, mixing samples with ELSA-ACTH (CIS bio international, Gif-sur-Yvette Cedex, France) in a 1,470 wizard γ-counter.
Insulin concentrations were analyzed by immunoradiometric assay (TFB, Tokyo, Japan). Plasma glucose levels were analyzed using a Roche/Hitachi Modular P analyzer by mixing samples with Gluco-quant Glucose/HK (Roche Diagnostics, Indianapolis, IN., USA). Epinephrine, norepinephrine and cortisol were assayed in duplicate by radioimmunoassay using commercial kits (BioSource Europe S.A., Belgium).
Body composition was analyzed using the Inbody 720 (Biospace. CO., Ltd., Seoul, Korea). All body composition parameters such as the body mass index (BMI), body fat mass, waist hip ratio and skeletal muscle mass were compared at baseline to measurements after 2-months of abstinence.
Using the fasting glucose and insulin data obtained, the Quantitative Insulin Sensitivity Check Index (QUICKI) and the Homeostasis Model Assessment-Insulin Resistance (HOMA-IR) were used to calculate insulin activity. Insulin secretion was assessed by the Homeostasis Model Assessment β-cell function (HOMA-β) from data obtained while in a fasting state.
The data were analyzed by nonparametric tests using SPSS 12.0 for Windows (SPSS Inc., Chicago, IL. USA). The Wilcoxon signed rank test was used to analyze the difference in the measures both before and after abstinence. A p-value lower than 0.05 was considered significant.
The mean age of the subjects was 39.0±7.8, with a range of 28-52 years of age. The mean age that the subjects began smoking was 18.6±1.7 with a range of 17-22 years of age. The mean number of cigarettes consumed per day was 19.3±1.7. The mean score for the FTND was 2.8±1.7.
Body weight (p=0.029) and waist circumference (p=0.020) increased during the two months of smoking cessation. Body fat composition increased more than muscle (p=0.013) (
LDL-C levels decreased significantly (p=0.028) after smoking cessation; however, changes in HDL-C, TC and TG were not significant (
During smoking cessation, both HOMA-β and HOMA-IR exhibited a significant increase while the QUICKI showed a significant decrease (p=0.047, p=0.005 and p=0.005 respectively). Overall, the insulin resistance and secretion increased and the insulin sensitivity decreased (
The data did not reveal any effects from short-term smoking abstinence on thyroid hormones; however, the T3 levels decreased (p=0.005) and the cortisol levels increased significantly (p=0.047) (
Previous studies have reported that smoking is associated with increased blood glucose concentrations, insulin resistance and impaired insulin sensitivity.
The results of this study showed that insulin resistance increased and insulin sensitivity decreased despite smoking cessation. This might have been due to weight gain, increased fat composition and increased cortisol after smoking cessation or the short duration of smoking cessation. After an extended time of smoking cessation, insulin resistance may decrease and insulin sensitivity may increase.
In addition, the results of this study showed that during smoking cessation, insulin secretion increased more than during smoking. High beta cell activity, after smoking cessation, is not consistent with the findings of some prior studies.
Smoking was positively associated with LDL-C in males of European, but not of African descent.
The biological mechanisms explaining the effect of smoking on lipid metabolism have not been fully elucidated. Some laboratories have demonstrated that hepatic lipase is increased in smokers,
Regardless of the conflicting study results, plasma enzymes appear to be involved in the metabolism of triglycerides and HDL-C, which are potentially affected by smoking. It is possible that the effect of smoking on these enzymes is dependent on numerous factors including: gender, age, genetic background, and the ethnicity of smokers. Other important factors include dietary habits, physical activity, and lifestyle as well as differences in public health awareness.
There have been very few studies examining the effect of smoking cessation on lipid profiles. In addition, the reported study results have been inconsistent. A meta-analysis of 27 studies concluded that HDL-C was significantly increased but that the levels of TC, LDL-C, and TG did not change significantly after smoking cessation.
Some possible explanations for the changes in the lipid profile caused by smoking cessation include the following. HDL-C normally promotes cholesterol clearance and decreases LDL-C oxidation.
In this study, LDL-C levels decreased after smoking cessation, suggesting that the beneficial effects of smoking cessation; however, changes in HDL-C and TC were not significant. The discrepancy between the levels of cholesterol in this study compared to previous studies may be due to the fact that subjects that stopped smoking included those with recent and long-term cessation, resulting in the confounding of short- and long-term changes in the cholesterol levels.
The limitations of this study include the following. First, the sample size was relatively small. Future studies will need to recruit more subjects in order to increase the statistical power of this research. Second, only males were recruited for this study, so the findings are not generalizable to women. Gender differences in smoking behaviors and metabolic brain responses to nicotine have been reported.
Despite these limitations, the strength of the present study is that the findings can be used to guide clinicians and patients in smoking cessation. It is important to include education about weight control with diet and regular exercise (both aerobic and anaerobic) for blood glucose control in smoking cessation programs.
In conclusion, the results of this study showed that during the initial stage of smoking cessation, insulin resistance increased and insulin sensitivity decreased due to elevated body weight and fat composition. Therefore, weight control including diet control and physical exercise is necessary as part of smoking cessation programs. In the short-term, the positive effects of quitting smoking might not be immediately obvious. Therefore, further longitudinal studies including the control of the BMI, physical activity and other confounding risk factors such as medical history and alcohol consumption are needed to evaluate the effects of smoking cessation on physical and laboratory findings.
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0016708). The Ministry of Education, Science and Technology had no further role in study design; in the collection, analysis and interpretation of data; in the writing of the report; and in the decision to submit the paper of publication.
Homeostasis Model Assessment-Insulin Resistance changes after smoking cessation.
Homeostasis Model Assessment β-cell function changes after smoking cessation.
Profile of participants before and after smoking cessation
P-value was obtained by Wilcoxon signed rank test on differences. *p<0.05. BMI: body mass index, WHR: waist hip ratio, BFM: body fat mass, SMM: skeletal muscle mass, FPG: fasting plasma glucose, HOMA-IR: homeostasis model assessment-insulin resistance, HOMA-β: homeostasis model assessment β-cell function, QUICKI: quantitative insulin sensitivity check index, TC: total cholesterol, TG: triglyceride, HDL-C: high density lipoprotein-cholesterol, LDL-C: low density lipoprotein-cholesterol, ACTH: adreno cortico tropic hormone, GH: growth hormone, Free T4: free thyroxine, T3: thyronine, TSH: thyroid-stimulating hormone