Serum N-Desmethylcitalopram Concentrations are Associated with the Clinical Response to Citalopram of Patients with Major Depression

Gul Ozbey; Berna Yucel; Nurdan Eren Bodur; Serap Erdogan Taycan; Tayyibe Arslan; Nazan Cerit; Nevzat Yuksel; Ismail Cuneyt Guzey; Canan Uluoglu

doi:10.30773/pi.2017.05.22

Psychiatry Investig > Volume 15(3); 2018 > Article

Ozbey, Yucel, Bodur, Taycan, Arslan, Cerit, Yuksel, Guzey, and Uluoglu: Serum N-Desmethylcitalopram Concentrations are Associated with the Clinical Response to Citalopram of Patients with Major Depression

Original Article

Psychiatry Investigation 2018;15(3):313-319.

Published online: February 22, 2018

DOI: https://doi.org/10.30773/pi.2017.05.22

Serum N-Desmethylcitalopram Concentrations are Associated with the Clinical Response to Citalopram of Patients with Major Depression

Gul Ozbey¹, Berna Yucel², Nurdan Eren Bodur³, Serap Erdogan Taycan⁴, Tayyibe Arslan⁵, Nazan Cerit⁶, Nevzat Yuksel⁷, Ismail Cuneyt Guzey^8,⁹, Canan Uluoglu¹⁰

¹Department of Pharmacology, Akdeniz University Medical Faculty, Antalya, Turkey

²Izmir State Authority, Izmir, Turkey

³Department of Psychiatry, Erenkoy Psychiatry and Neurology Education and Research Hospital, Istanbul, Turkey

⁴Haydarpasa Numune State Hospital for Research and Training, Istanbul, Turkey

⁵Department of Psychiatry, Balıkesir State Hospital, Balıkesir, Turkey

⁶Family Practise Office 7, Antalya, Turkey

⁷Department of Psychiatry, Gazi University Medical Faculty, Ankara, Turkey

⁸Department of Research and Development, Division of Psychiatry, St Olavs University Hospital, Trondheim, Norway

⁹Department of Neuroscience, Faculty of Medicine, Norwegian University of Science and Technology, Trondheim, Norway

¹⁰Department of Pharmacology, Gazi University Medical Faculty, Ankara, Turkey

Correspondence: Gul Ozbey, MD Department of Pharmacology, Akdeniz University Medical Faculty, Dumulpinar Avenue, Antalya 07070, Turkey Tel: +90 242 2496925, Fax: +90 242 2274482, E-mail: gulozbey@akdeniz.edu.tr

Received February 8, 2017 Revised April 14, 2017 Accepted May 22, 2017

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective

Citalopram (CITA) is a widely used and well-tolerated selective serotonin reuptake inhibitor. The aim of the study was to evaluate the possible influences of serum concentrations of CITA and its major metabolite n-desmethylcitalopram (NDCITA) on the efficacy and tolerability of CITA in patients with major depressive disorder.

Methods

The study included 46 outpatients with major depressive disorder who received CITA. The efficacy and tolerability were assessed for 6 weeks. Serum CITA and NDCITA levels were measured at the 4th week.

Results

The HDRS17 total scores of the patients with high NDCITA and CITA & NDCITA concentrations showed a more significant reduction compared to the patients with expected and low serum NDCITA and CITA & NDCITA concentrations. However, we did not observe a correlation between the serum concentrations and the side effects of CITA, NDCITA, and CITA & NDCITA.

Conclusion

Our results suggested the potential contribution of NDCITA to the antidepressant effect of CITA. Further studies involving larger clinical samples are required to confirm the impact of serum NDCITA concentrations on the efficacy of CITA.

Keywords: Serum concentration, Citalopram, N-desmethylcitalopram, Efficacy, Tolerability, Major depression

INTRODUCTION

Major depressive disorder (MDD) is the most prevalent psychiatric disease with a highly variable treatment response to antidepressants [1,2], with up to one-third of patients not achieving an adequate response to the first-line antidepressant treatment [3]. Selective serotonin reuptake inhibitors (SSRIs) are the first choice in the pharmacological treatment of most cases of depression and their therapeutic window has not been established. Large inter-individual variations in the pharmacokinetics and pharmacodynamics of antidepressants, requires the development of adequate tools for optimizing treatment a major aim for psychopharmacology research [4].

Citalopram (CITA) as a racemic drug (R, S-citalopram) is a widely used and well-tolerated antidepressant that belongs to the SSRI class [5]. CITA is metabolized to n-desmethylcitalopram (NDCITA) and n-didesmethylcitalopram (NDDCITA) through n-demethylation [6,7]. The concentrations of these metabolites at steady state in the plasma in relation to the CITA levels are 30-50% for NDCITA and 5-10% for NDDCITA [8]. With a wide inter-individual variation, the plasma half-life (t_1/2) at steady state is approximately 35 hours for CITA, 50 hours for NDCITA, and 100 hours for NDDCITA [8]. Although in vitro studies suggested that CITA is mainly responsible for the antidepressant effect, NDCITA also has affinity for the human serotonin transporter that cannot be ignored, especially at higher serum concentrations [9].

As for most SSRIs, the therapeutic window of CITA hasn’t been established yet. Although an earlier study recommended a 30 ng/mL steady-state serum concentration as a lower limit [10], two recent studies suggested that 50 ng/mL of serum CITA concentrations are required at steady state for efficient therapy [11,12]. However, studies that examine the effects of serum NDCITA concentrations on the clinical response to CITA are lacking in the literature. The aim of the current study was to evaluate the possible influences of serum concentrations of CITA and NDCITA on the efficacy and tolerability of CITA in MDD patients.

Methods

Subjects and treatment

The subjects of this study (n=46) were part of a previously reported study to investigate the effects of ABCB1 C3435T polymorphism on susceptibility to depression and the clinical response to CITA [13]. The study was conducted in a prospective and open-label style between 2006 and 2008 at the Gazi University Medical Faculty Hospital, Ankara, Turkey. Patients who applied to the psychiatric outpatient clinic and met the Diagnostic and Statistical Manual of Mental Disorders Fourth Edition (DSM-IV) criteria for MDD were included in the study. The exclusion criteria included clinically significant laboratory abnormalities; major physical and neurological illnesses; psychiatric disorders except for major depression; substance abuse and dependence, except for nicotine dependence; pregnancy; breastfeeding; using any antidepressant drug within the previous 12 months; being younger than 18 and older than 65; and treatment with concomitant psychotropic drugs, except for hydroxyzine for insomnia. All participants were biologically unrelated Turkish subjects and were recruited from the same geographical area (Ankara region of Turkey). The study was approved by the Human Ethics Committee of the Gazi University Medical Faculty, according to the 2004 Helsinki Declaration (approval number, 271; date, 25.09.2006). Patients were informed about the study and they gave their written informed consent.

CITA was administered at an initial dose of 20 mg/day, with the possibility of adjustment up to 30 mg/day according to the therapeutic response. All patients affirmed an intake of CITA once a day between 8:00 and 9:00 p.m. every day during the study. Blood samples were taken from 8:00-9:00 a.m. (12 hours after the drug administration) at the 4th week for the measurement of steady-state drug concentrations. Protocol visits were completed at baseline and at the 1st, 2nd, 4th, and 6th weeks. The therapeutic response to CITA was evaluated by psychiatrists using the 17-item Hamilton Rating Scale for Depression (HDRS₁₇) at baseline and at the 1st, 2nd, 4th, and 6th weeks. The responders were defined as patients who demonstrated a 50% reduction from the baseline HDRS₁₇ total score at the 6th week. Tolerability assessments were conducted based on a query of side effects (dry mouth, nausea, constipation, palpitation, dizziness, increased perspiration, itching, headache, tremor, blurred vision, difficulty sleeping, sleeping too much, loss of sexual desire, poor concentration) at the 1st, 2nd, and 6th weeks by psychiatrists.

Analytical methods

Venous blood samples were drawn (10 mL) into the tubes that did not contain any additives, were centrifuged for 10 minutes at 3000 g immediately, and serum samples were stored at -20°C.

The serum concentrations of CITA and NDCITA were analyzed by an isocratic reversed-phase high-performance liquid chromatography (HPLC) method with ultraviolet detection (Agilent LC Systems, Series 1100, Agilent Technologies Inc., Santa Clara, CA, USA) as previously described [14]. The lowest limit of quantification was 5.0 ng/mL for both analytes. Coefficients of accuracy and precision for citalopram and NDCITA were 6.6, 92.1, 6.5, and 89.5%, respectively.

Statistical analysis

Statistical analysis focused on the comparison of three groups, which were defined according to the median CITA, NDCITA, and the sum of the CITA & NDCITA concentrations of patients. Patients with 25% above the median concentrations were defined as the “high CITA group” (>136.0 ng/mL), the “high NDCITA group” (>73.25 ng/mL), and the “high CITA & NDCITA group” (>197.5 ng/mL); those with 25% below the median concentrations were defined as the “low CITA group” (<86.0 ng/mL), the “low NDCITA group” (<42.75 ng/mL), and the “low CITA & NDCITA group” (<136.3 ng/mL); and those within the reference medians ±25% were defined as the “expected CITA group” (86.0-136.0 ng/mL), the “expected NDCITA group” (73.25-42.75 ng/mL), and the “expected CITA & NDCITA group” (136.3-197.5 ng/mL). The comparison of age, baseline HDRS₁₇ total score, CITA dose, serum CITA, NDCITA, and CITA & NDCITA concentrations among the groups were performed by one-way ANOVA. Male/female ratios among the groups were compared by the chi-squared test. Changes in the groups’ reduction in HDRS₁₇ total scores during the 6th week of the study were analyzed with repeated measures ANOVA. A comparison of the side effects among the groups was performed with Fisher’s exact test. The statistical significance was considered to be p<0.05. Statistical analyses were performed using the SPSS version 18.0 and Graph-Pad Prism version 5.

Results

Of the 54 patients included in the study, 8 patients dropped out due to the adverse effects or refusal of regular visits. Thus, 46 subjects of the 54 enrolled had sufficient data to analyze. The demographic characteristics; mean HDRS₁₇ total scores at baseline; CITA doses (mg/day); serum CITA, NDCITA, and CITA & NDCITA concentrations (ng/mL); and CITA, NDCITA, and CITA & NDCITA concentration/dose ratios (ng/mL/mg/day) as described above are shown in Table 1. No significant differences were found among the groups according to serum concentrations of CITA, NDCITA, and CITA & NDCITA for age (F=0.4866, p=0.6180; F=1.663, p=0.2015; F=1.125, p=0.3341; respectively), sex (χ²=2.008, df=2, p=0.3664; χ²=0.5567, df=2, p=0.7570; χ²=1.231, df=2, p=0.5405; respectively), and the baseline HDRS₁₇ total scores (F=0.3315, p=0.7197; F=1.097, p=0.3430; F=0.8598, p=0.4304; respectively). However, among the three groups of patients, significant differences were found in the serum concentrations and concentration/dose ratios of CITA (F=49.48, p<0.0001; F=39.52, p<0.0001; respectively), NDCITA (F=47.63, p<0.0001; F=35.92, p<0.0001; respectively), and CITA & NDCITA (F=39.84, p<0.0001; F=19.33, p<0.0001; respectively). Although the CITA dose was differed significantly among the groups for serum CITA (F=4.285, p=0.0201) and NDCITA (F=9.620, p=0.0004) concentrations, no significant differences were found among the groups for serum CITA & NDCITA concentrations (F=1.122, p=0.3351).

Serum concentrations and efficacy

The efficacy of CITA was evaluated by HDRS₁₇ total scores for patients with low, expected, and high serum CITA and NDCITA concentrations for 6 weeks. Although the HDRS₁₇ total scores of patients did not differ significantly among the groups for serum CITA concentrations (F=1.576, p=0.152) (Table 2), patients with high NDCITA and CITA & NDCITA concentrations showed a more significant reduction in HDRS₁₇ total scores during the 6 week than did patients with expected and low serum NDCITA and CITA & NDCITA concentrations (F=3.717, p=0.002; F=3.523, p=0.003, respectively) (Table 2).

Serum concentrations and tolerability

The tolerability of CITA was examined for patients with low, expected and high serum CITA, NDCITA, and CITA & NDCITA concentrations in reporters and non-reporters of side effects. The low, expected, and high serum concentration groups did not differ with respect to the reported side effects for CITA, NDCITA, and CITA & NDCITA concentrations (Table 3).

Discussion

The present results provide the first evidence that the HDRS₁₇ total scores of the patients with high NDCITA concentrations show a more significant reduction compared to the patients with expected and high serum NDCITA concentrations in MDD patients. However, HDRS₁₇ total scores were not associated with serum CITA concentrations. In addition, no correlation was observed between the serum CITA and serum NDCITA concentrations and adverse reactions.

Previous studies have revealed that the steady-state concentrations of CITA predict the antidepressant response of CITA in MDD patients. Although Ostad Haji et al. suggested that achieving approximately 50 ng/mL serum concentrations for CITA on day 7 is necessary for clinical improvement [11,12], an earlier study concluded that the lower limit of effective serum CITA concentrations was 30 ng/mL after day 7 [10]. In contrast to previous results, in this study, only 1 patient had a serum CITA concentration below 50 ng/mL (42 ng/mL), and we did not observe any relationship between the efficacy and serum CITA concentrations according to the patient groups with low (<86.0 ng/mL), expected (86.0-136.0 ng/mL), and high (>136.0 ng/mL) CITA concentrations at the 4th week. We measured relatively higher median serum CITA concentrations (101 ng/mL) compared to earlier reports than in the reports of previous results [11,12,15-17]. The difference in the serum CITA concentrations of this study might be explained in part by the sampling time for citalopram, taking comedications and CYP2C19 polymorphisms. Because citalopram is extensively metabolized by CYP2C19 [18], CYP2C19 polymorphisms, and the use of CYP2C19 inhibitors, especially proton pump inhibitors could increase the citalopram concentrations [19,20].

This is one of the first studies to exhibit a potential relationship between the efficacy of CITA and serum NDCITA concentrations. We found a more significant reduction in the HDRS₁₇ total scores in patients with high NDCITA concentrations (>73.25 ng/mL) than in patients with expected (73.25-42.75 ng/mL) and low serum NDCITA concentrations (<42.75 ng/mL). Although the previous clinical studies ignored the contribution of NDCITA to the antidepressant effect of CITA, the human serotonin transporter affinity of NDCITA [21] with a long plasma half-life (50 hours) emphasizes the possible contribution to the effect especially at high serum concentrations as seen in this study. Further studies are needed to examine the potential relationship between the serum NDCITA concentrations and the antidepressant effect of CITA in larger clinical samples.

As the therapeutic drug monitoring (TDM) studies have focused only on evaluating the relevance of serum concentrations on therapeutic response, the tolerability-serum concentration relationship has been ignored [22]. In the present study, we also evaluated whether the serum CITA and NDCITA concentrations were associated with the side effects of CITA. No associations were observed between the serum CITA and NDCITA concentrations and any side effects. Although our findings show that there is the lack of a relationship between the serum CITA and NDCITA concentrations and the tolerability for CITA, the sample size of the study is not large enough to examine whether the tolerability-serum concentration interaction exists decisively.

This study had some limitations regarding the sample size and the time of the blood sampling. The lack of CYP2C19 genotyping is a major limitation of the study. The number of patients was relatively small for examining the relationship between serum concentrations and efficacy and tolerability. In addition, the blood samples for the measurements of serum concentrations were drawn at steady-state but did not represent trough concentrations.

Despite these limitations, the present study showed that serum concentrations of NDCITA have an impact on the efficacy but not on the tolerability of CITA. In addition, serum concentrations of CITA were not associated with either efficacy or with the tolerability of the drug. The results of this study provide the first evidence for the potential contribution of NDCITA to the antidepressant effect of CITA at higher serum concentrations of the drug. The studies in larger clinical samples, at multiple dose levels, and also including trough serum concentrations of CITA and NDCITA are required to confirm whether the impact of serum CITA and NDCITA concentrations on the efficacy and tolerability of CITA.

ACKNOWLEDGEMENTS

This study was supported by grants from the Scientific Research Projects of Gazi University (Project No: 01/2004-44).

Table 1.

Patient characteristics according to serum CITA, NDCITA, and CITA & NDCITA concentrations

Patient characteristics	ng/mL	Groups according to serum concentrations
		CITA			NDCITA			CITA&NDCITA
		Low	Expected	High	Low	Expected	High	Low	Expected	High
		<86	86-136	>136	<42.8	42.8-73.3	>73.3	<136.3	136.3-197.5	>197.5
N	46	11	22	13	11	24	11	11	24	11
Age	39.4±13.6	41.7±11.6	39.9±12.8	36.4±16.6	38.0±13.7	43.0±14.4	33.8±10.1	38.1±13.0	42.0±13.3	34.8±14.6
	(18-65; 37)	(30-62; 37)	(18-64; 37)	(20-65; 31)	(22-62; 32)	(18-65; 42)	(20-51; 35)	(24-62; 32)	(18-65; 42)	(20-63; 34)
		F=0.4866, p=0.6180			F=1.663, p=0.2015			F=1.125, p=0.3341
Sex	37/9	9/2	16/6	12/1	9/2	20/4	8/3	10/1	18/6	9/2
		χ²=2.008, df=2, p=0.3664			χ²=0.5567, df=2, p=0.7570			χ²=1.231, df=2, p=0.5405
HDRS_17b	17.3±4.6	17.8±5.9	17.3±4.7	16.3±3.6	17.0±3.9	16.4±4.9	18.9±4.8	18.6±5.8	16.4±4.6	17.3±3.4
	(10-33; 17)	(12-33; 17)	(10-26; 17)	(12-26; 17)	(12-24; 17)	(10-33; 17)	(12-26; 17)	(12-33; 18)	(10-26; 16)	(12-26; 17)
		F=0.3315, p=0.7197			F=1.097, p=0.3430			F=0.8598, p=0.4304
CITA dose	27.2±4.6	25.5±5.2	29.1±2.9	25.4±5.2	22.7±4.7	28.8±3.4	28.2 ± 4.1	25.5±5.2	27.5±4.4	28.2±4.1
	(20-30; 30)	(20-30; 30)	(20-30; 30)	(20-30; 30)	(20-30; 20)	(20-30; 30)	(20-30; 30)	(20-30; 30)	(20-30; 30)	(20-30; 30)
		F=4.285, p=0.0201^*			F=9.620, p=0.0004^***			F=1.122, p=0.3351
Concentrations		69.4±12.3	101.9±9.2	160.5±40.5	25.4±9.4	59.8±8.1	92.3±29.5	109.2±22.4	164.6±16.4	242.4±40.6
		(42-83; 74)	(87-121; 101)	(136-280; 144)	(12-42; 28)	(43-73; 62)	(74-179; 83)	(70-134; 117)	(137-196; 165)	(202-336; 226)
		F=49.48, p<0.0001^***			F=47.63, p<0.0001^***			F=39.84, p<0.0001^***
C/D ratio		2.8±0.7	3.6±0.7	6.5±1.8	1.1±0.4	2.1±0.4	3.4±1.1	4.4±0.7	6.2±1.5	8.9±2.4
		(2.0-4.1; 2.6)	(2.9-6.1; 3.4)	(4.5-10.2; 6.8)	(0.6-1.8; 1.1)	(1.4-2.8; 2.2)	(2.5-6.0; 2.9)	(3.4-5.9; 4.4)	(4.7-9.8; 5.7)	(6.7-14.2; 7.6)
		F=39.52, p<0.0001^***			F=35.92, p<0.0001^***			F=19.33, p<0.0001^***

Age, HDRS_17b, CITA dose (mg/day), serum concentrations, and concentration/dose ratios (C/D) values are expressed as mean±SD (range; median), and sex values expressed as number; one way ANOVA (analysis of variance) and chi-square test (χ²) was applied for the comparisons.

^* p<0.05,

^*** p<0.001.

CITA: citalopram, NDCITA: n-desmethylcitalopram, CITA & NDCITA: citalopram and n-desmethylcitalopram, HDRS_17b, baseline hamilton depression rating scale 17, SD: standart deviation, NS: nonsignificant

Table 2.

HDRS₁₇ scores of patients during the study according to serum CITA, NDCITA, and CITA & NDCITA concentrations

HDRS₁₇ scores	CITA concentrations			NDCITA concentrations			CITA & NDCITA concentrations
HDRS₁₇ scores	Low	Expected	High	Low	Expected	High	Low	Expected	High
N	11	22	13	11	24	11	11	24	11
HDRS₁₇ baseline	17.8±1.8	17.3±1.0	16.3±1.0	17.0±1.2	16.4±1.0	18.9±1.5	18.6±1.7	16.4±1.0	17.3±1.0
HDRS₁₇ 1st week	12.8±1.3	14.1±1.2	10.9±1.2	12.5±1.0	13.1±1.0	12.8±2.0	13.8±1.3	13.6±1.1	10.5±1.5
HDRS₁₇ 2nd week	8.4±1.4	10.4±1.5	8.5±1.1	7.0±0.9	9.3±1.0	11.9±2.5	8.2±1.4	10.7±1.3	7.6±1.4
HDRS₁₇ 4th week	7.3±2.0	6.9±1.0	5.4±1.2	6.2±0.9	6.2±1.2	7.6±1.7	8.5±1.8	6.1±1.0	5.5±1.4
HDRS₁₇ 6th week	6.5±1.8	5.2±0.9	4.1±1.2	5.3±1.1	5.8±1.1	4.0±1.5	7.0±1.7	5.0±0.9	4.0±1.4
	F=1.227, p=0.293			F=3.717, p=0.002^*			F=3.523, p=0.003^*

Repeated measures analysis of variance was applied for the comparisons.

^* p<0.05.

HDRS_17b: baseline hamilton depression rating scale 17, CITA: citalopram, NDCITA: n-desmethylcitalopram, CITA & NDCITA: citalopram and n-desmethylcitalopram, HDRS₁₇: hamilton depression rating scale 17

Table 3.

Side effects of patients during the study according to serum CITA, NDCITA, and CITA & NDCITA concentrations

Side effects	CITA			NDCITA			CITA & NDCITA
Side effects	R	NR	p values	R	NR	p values	R	NR	p values
Dry mouth			0.744			1.000			1.000
Low-expected	17	16		19	16		19	16
High	8	5		6	5		6	5
Nause			0.185			0.320			0.092
Low-expected	16	17		16	19		17	18
High	3	10		3	8		2	9
Constipation			0.435			1.000			1.000
Low-expected	6	27		8	27		8	27
High	4	9		2	9		2	9
Palpitation			0.867			1.000			1.000
Low-expected	3	30		6	29		5	30
High	4	9		1	10		2	9
Dizziness			1.000			0.410			1.000
Low-expected	7	26		9	26		8	27
High	3	10		1	10		2	9
Increased perspiration			1.000			0.491			1.000
Low-expected	17	16		19	16		18	17
High	6	7		4	7		5	6
Itching			0.565			1.000			1.000
Low-expected	2	31		3	32		3	32
High	2	11		1	10		1	10
Headache			0.512			1.000			0.169
Low-expected	13	20		15	20		13	22
High	7	6		5	6		7	4
Tremor			0. 565			0.238			1.000
Low-expected	2	31		2	33		3	32
High	2	11		2	9		1	10
Blurred vision			0.410			1.000			0.421
Low-expected	8	25		7	28		8	27
High	1	12		2	9		1	10
Difficulty sleeping			1.000			0.619			0.619
Low-expected	4	29		4	31		4	31
High	2	11		2	9		2	9
Sleeping too much			1.000			0.296			1.000
Low-expected	13	20		12	23		14	21
High	5	8		6	5		4	7
Loss of sexual desire			0.410			0.664			1.000
Low-expected	8	25		6	29		7	28
High	1	12		3	8		2	9
Poor concentration			0.461			1.000			0.242
Low-expected	10	23		9	26		11	24
High	2	11		3	8		1	10

Fisher’s exact test applied for the comparisons. CITA: citalopram, NDCITA: n-desmethylcitalopram, CITA & NDCITA: citalopram and n-desmethylcitalopram, R: reporter, NR: nonreporter

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